Intestinal drug absorption is one of several factors that determine the success of oral drug products and their efficacy, toxicity, and pharmacokinetics. Therefore, models that predict intestinal drug absorption are becoming increasingly important in early drug development to accelerate identifying promising or problematic compounds. Since permeability is one of the factors that affect drug absorption, it has become the focus of several experimental models available to researchers. The use of cell culture provides a method for predicting drug permeability by using the cell monolayer in the dual-chamber diffusion system to simulate the process of drug entering the blood from the intestinal lumen. Among them, the Caco-2 model and MDR1-MDCK model are widely used. https://www.creative-bioarray.com/Services/mdck-permeability-assay.htm